這是一款(kuan)無血(xue)清(qing)即用(yong)型細(xi)胞(bao)(bao)保(bao)(bao)存(cun)液(ye)(ye)(ye)(ye)，比(bi)較大(da)的特點就是無需程序(xu)降溫盒(he)及稀釋，無需分步(bu)降溫，直(zhi)接使用(yong)！可在-80℃長(chang)期保(bao)(bao)存(cun)ES/iPS細(xi)胞(bao)(bao)、腫瘤細(xi)胞(bao)(bao)和常(chang)規細(xi)胞(bao)(bao)。冷(leng)凍(dong)(dong)(dong)(dong)細(xi)胞(bao)(bao)時(shi)，用(yong)1ml該(gai)細(xi)胞(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)液(ye)(ye)(ye)(ye)懸浮處(chu)于對(dui)數生長(chang)期的細(xi)胞(bao)(bao)，不(bu)(bu)需預(yu)冷(leng)，直(zhi)接-80℃冷(leng)凍(dong)(dong)(dong)(dong)保(bao)(bao)存(cun)，也可用(yong)液(ye)(ye)(ye)(ye)氮(dan)快速(su)冷(leng)凍(dong)(dong)(dong)(dong)細(xi)胞(bao)(bao)；復(fu)蘇時(shi)用(yong)恒溫箱或者水浴鍋快速(su)復(fu)蘇細(xi)胞(bao)(bao)即可。不(bu)(bu)僅操作方便，更(geng)能提(ti)供穩(wen)定(ding)的凍(dong)(dong)(dong)(dong)存(cun)效(xiao)果，獲得更(geng)好的細(xi)胞(bao)(bao)活力。一款(kuan)不(bu)(bu)需要放液(ye)(ye)(ye)(ye)氮(dan)罐也能長(chang)期保(bao)(bao)存(cun)的細(xi)胞(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)液(ye)(ye)(ye)(ye)試用(yong)裝申(shen)請(qing)正在進(jin)行。。。無血(xue)清(qing)細(xi)胞(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)液(ye)(ye)(ye)(ye)用(yong)于其他類(lei)型哺(bu)乳動物細(xi)胞(bao)(bao)的儲存(cun)。蘇州(zhou)無血(xue)清(qing)細(xi)胞(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)液(ye)(ye)(ye)(ye)廠家現貨

產品(pin)特性(xing)：a.可(ke)直接放(fang)置(zhi)(zhi)于(yu)-80℃冰(bing)箱，無(wu)(wu)需降(jiang)溫(wen)，節省時間和(he)精力(li)；b.即用(yong)型，無(wu)(wu)需現配，操(cao)(cao)作(zuo)(zuo)方(fang)(fang)便，可(ke)減少實驗中因操(cao)(cao)作(zuo)(zuo)引起(qi)的污染；c.在多種哺乳細(xi)(xi)胞(bao)(bao)(bao)系中經過性(xing)能(neng)驗證，其復蘇(su)率(lv)和(he)活率(lv)達90%以上；d.可(ke)長期存儲于(yu)-80℃冰(bing)箱(5年(nian)以上)，取用(yong)方(fang)(fang)便；e.采用(yong)成分明確的無(wu)(wu)血清(qing)配方(fang)(fang)，價格(ge)比常規(gui)自配細(xi)(xi)胞(bao)(bao)(bao)凍(dong)(dong)存液更為低廉(lian)；保(bao)(bao)存溫(wen)度(du)：2~8℃具體操(cao)(cao)作(zuo)(zuo)步(bu)驟(zou)：1、培(pei)養皿或(huo)培(pei)養瓶中細(xi)(xi)胞(bao)(bao)(bao)按常規(gui)方(fang)(fang)法消化，或(huo)分離(li)(li)獲得原代細(xi)(xi)胞(bao)(bao)(bao)后(hou)，收集于(yu)離(li)(li)心管中。2、使用(yong)離(li)(li)心機離(li)(li)心，去除(chu)上清(qing)，收集細(xi)(xi)胞(bao)(bao)(bao)沉淀。3、根據細(xi)(xi)胞(bao)(bao)(bao)生(sheng)長密(mi)(mi)度(du)加入適量(liang)的細(xi)(xi)胞(bao)(bao)(bao)凍(dong)(dong)存液進行重(zhong)懸，充分混勻(推薦凍(dong)(dong)存密(mi)(mi)度(du)為1-2×106/ml)。4、將細(xi)(xi)胞(bao)(bao)(bao)懸液分裝至(zhi)凍(dong)(dong)存管中，直接放(fang)置(zhi)(zhi)于(yu)-80℃冰(bing)箱保(bao)(bao)存。24小時后(hou)可(ke)移入液氮長期保(bao)(bao)存(可(ke)選(xuan))。長沙無(wu)(wu)血清(qing)細(xi)(xi)胞(bao)(bao)(bao)凍(dong)(dong)存液服(fu)務電(dian)話無(wu)(wu)血清(qing)細(xi)(xi)胞(bao)(bao)(bao)凍(dong)(dong)存液產品(pin)特色(se)：各批產品(pin)之(zhi)間有更高的產品(pin)質量(liang)一致性(xing)。

無血(xue)清細(xi)胞(bao)(bao)(bao)凍(dong)存(cun)(cun)液(ye)復蘇(su)細(xi)胞(bao)(bao)(bao)實驗步驟：1.從(cong)冰箱里取(qu)出細(xi)胞(bao)(bao)(bao)冷凍(dong)保存(cun)(cun)管(guan)(guan)(guan)，立即放入(ru)37℃振動水(shui)浴槽中(zhong)快(kuai)速解凍(dong)。2.待凍(dong)存(cun)(cun)管(guan)(guan)(guan)中(zhong)細(xi)胞(bao)(bao)(bao)混(hun)合(he)(he)液(ye)完(wan)全(quan)融化后，立即加入(ru)1ml細(xi)胞(bao)(bao)(bao)培養(yang)基(ji)于該(gai)(gai)冷凍(dong)管(guan)(guan)(guan)中(zhong)與細(xi)胞(bao)(bao)(bao)混(hun)合(he)(he)，再將細(xi)胞(bao)(bao)(bao)混(hun)合(he)(he)液(ye)從(cong)凍(dong)存(cun)(cun)管(guan)(guan)(guan)中(zhong)移入(ru)含有9ml該(gai)(gai)細(xi)胞(bao)(bao)(bao)培養(yang)基(ji)的試管(guan)(guan)(guan)中(zhong)，混(hun)合(he)(he)均勻(yun)。3.離心(xin)收集培養(yang)細(xi)胞(bao)(bao)(bao)（參考離心(xin)條件：1,000~2,000rpm，4℃，3~5min），移去上清液(ye)。4.清洗(xi)細(xi)胞(bao)(bao)(bao)，充分洗(xi)凈殘留(liu)凍(dong)存(cun)(cun)液(ye)。5.加入(ru)適量的新鮮細(xi)胞(bao)(bao)(bao)培養(yang)基(ji)，使用(yong)移液(ye)管(guan)(guan)(guan)緩(huan)緩(huan)地均勻(yun)細(xi)胞(bao)(bao)(bao)混(hun)合(he)(he)液(ye)。適量地稀釋后，將細(xi)胞(bao)(bao)(bao)混(hun)合(he)(he)液(ye)移至事先準(zhun)備好的培養(yang)瓶中(zhong)。

無(wu)血清快(kuai)速(su)細(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)(cun)(cun)(cun)液(ye)保存(cun)(cun)(cun)(cun)(cun)(cun)條(tiao)件：儲存(cun)(cun)(cun)(cun)(cun)(cun)于(yu)(yu)4℃以(yi)下(xia)(xia)。質量保障期從(cong)產(chan)品的(de)(de)生(sheng)產(chan)日期起(qi)，為(wei)期2年(nian)。3個月以(yi)上(shang)沒有使用(yong)凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)(cun)(cun)(cun)液(ye)計(ji)劃時(shi)，盡可(ke)(ke)能-20℃冷(leng)凍(dong)(dong)(dong)(dong)保存(cun)(cun)(cun)(cun)(cun)(cun)。為(wei)避免重復(fu)凍(dong)(dong)(dong)(dong)融過(guo)程可(ke)(ke)能導(dao)致的(de)(de)凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)(cun)(cun)(cun)液(ye)品質下(xia)(xia)降(jiang)和(he)(he)性能降(jiang)低，推薦將(jiang)產(chan)品分(fen)(fen)裝(zhuang)成(cheng)小管(guan)(guan)后(hou)，再(zai)存(cun)(cun)(cun)(cun)(cun)(cun)放于(yu)(yu)4℃以(yi)下(xia)(xia)或-20℃冰箱凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)(cun)(cun)(cun)。無(wu)血清快(kuai)速(su)細(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)(cun)(cun)(cun)液(ye)細(xi)(xi)胞(bao)(bao)(bao)(bao)冷(leng)凍(dong)(dong)(dong)(dong)保存(cun)(cun)(cun)(cun)(cun)(cun)方(fang)法：選擇凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)(cun)(cun)(cun)處于(yu)(yu)對數(shu)生(sheng)長期的(de)(de)細(xi)(xi)胞(bao)(bao)(bao)(bao)有助于(yu)(yu)提高復(fu)蘇(su)細(xi)(xi)胞(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)活率。1、按(an)照常用(yong)方(fang)法收集(ji)懸浮細(xi)(xi)胞(bao)(bao)(bao)(bao)或貼壁細(xi)(xi)胞(bao)(bao)(bao)(bao)于(yu)(yu)試(shi)管(guan)(guan)中。2、按(an)照培養細(xi)(xi)胞(bao)(bao)(bao)(bao)密度(du)和(he)(he)所(suo)用(yong)細(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)(cun)(cun)(cun)管(guan)(guan)的(de)(de)尺(chi)寸計(ji)算所(suo)需(xu)凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)(cun)(cun)(cun)細(xi)(xi)胞(bao)(bao)(bao)(bao)數(shu)。3、取(qu)相當于(yu)(yu)所(suo)需(xu)細(xi)(xi)胞(bao)(bao)(bao)(bao)數(shu)的(de)(de)細(xi)(xi)胞(bao)(bao)(bao)(bao)懸浮液(ye)量，置于(yu)(yu)離(li)(li)心(xin)(xin)管(guan)(guan)中，離(li)(li)心(xin)(xin)收集(ji)培養細(xi)(xi)胞(bao)(bao)(bao)(bao)（參考離(li)(li)心(xin)(xin)條(tiao)件：1,000~2,000rpm，4℃，3~5min）。移去離(li)(li)心(xin)(xin)管(guan)(guan)中的(de)(de)上(shang)清液(ye)。4、加入(ru)適量的(de)(de)無(wu)血清型細(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)(cun)(cun)(cun)液(ye)于(yu)(yu)離(li)(li)心(xin)(xin)管(guan)(guan)中，使細(xi)(xi)胞(bao)(bao)(bao)(bao)濃度(du)為(wei)5×105至5×106cells/ml。緩(huan)慢地(di)混(hun)合(he)均勻，制成(cheng)細(xi)(xi)胞(bao)(bao)(bao)(bao)混(hun)合(he)液(ye)。5、將(jiang)離(li)(li)心(xin)(xin)管(guan)(guan)中的(de)(de)細(xi)(xi)胞(bao)(bao)(bao)(bao)混(hun)合(he)液(ye)分(fen)(fen)裝(zhuang)于(yu)(yu)已標(biao)示完全(quan)的(de)(de)冷(leng)凍(dong)(dong)(dong)(dong)保存(cun)(cun)(cun)(cun)(cun)(cun)管(guan)(guan)中。6、直接將(jiang)含(han)細(xi)(xi)胞(bao)(bao)(bao)(bao)混(hun)合(he)液(ye)的(de)(de)凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)(cun)(cun)(cun)管(guan)(guan)放入(ru)-70℃以(yi)下(xia)(xia)冰箱，長期冷(leng)凍(dong)(dong)(dong)(dong)保存(cun)(cun)(cun)(cun)(cun)(cun)。7、若研究者需(xu)要液(ye)氮保存(cun)(cun)(cun)(cun)(cun)(cun)時(shi)，可(ke)(ke)將(jiang)完全(quan)凍(dong)(dong)(dong)(dong)結(jie)的(de)(de)凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)(cun)(cun)(cun)管(guan)(guan)（放入(ru)-70℃以(yi)下(xia)(xia)冰箱后(hou)至少(shao)一晝(zhou)夜）移至-196℃液(ye)氮罐。無(wu)血清細(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)(cun)(cun)(cun)液(ye)產(chan)品性能：傳統的(de)(de)凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)(cun)(cun)(cun)液(ye)，一般由胎牛血清、DMSO等組分(fen)(fen)組成(cheng)。

對于很多科(ke)研(yan)汪(wang)來說，細(xi)(xi)(xi)(xi)胞(bao)(bao)是脆弱(ruo)又(you)重要(yao)的(de)(de)(de)寶(bao)貝之一(yi)，承擔著(zhu)重要(yao)的(de)(de)(de)實驗數據希望(wang)。尤(you)其是在凍存復(fu)蘇(su)的(de)(de)(de)時候，經常會(hui)(hui)因為(wei)一(yi)些小細(xi)(xi)(xi)(xi)節(jie)導(dao)致問(wen)題。比如：沒(mei)有控(kong)制(zhi)好細(xi)(xi)(xi)(xi)胞(bao)(bao)的(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)長(chang)密(mi)(mi)(mi)度(du)(du)細(xi)(xi)(xi)(xi)胞(bao)(bao)的(de)(de)(de)密(mi)(mi)(mi)度(du)(du)對細(xi)(xi)(xi)(xi)胞(bao)(bao)的(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)存質量(liang)有著(zhu)重要(yao)的(de)(de)(de)影響(xiang)，畢(bi)竟它們是一(yi)群又(you)不(bu)能(neng)擠著(zhu)了(le)也不(bu)能(neng)孤獨的(de)(de)(de)嬌貴(gui)寶(bao)寶(bao)。密(mi)(mi)(mi)度(du)(du)過(guo)高會(hui)(hui)讓(rang)細(xi)(xi)(xi)(xi)胞(bao)(bao)沒(mei)有足(zu)夠的(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)存空間，密(mi)(mi)(mi)度(du)(du)過(guo)低會(hui)(hui)讓(rang)細(xi)(xi)(xi)(xi)胞(bao)(bao)無法互(hu)相連接健(jian)康(kang)生(sheng)(sheng)(sheng)(sheng)長(chang)。建議大家在細(xi)(xi)(xi)(xi)胞(bao)(bao)接種前，一(yi)定(ding)要(yao)調整好適合(he)細(xi)(xi)(xi)(xi)胞(bao)(bao)生(sheng)(sheng)(sheng)(sheng)長(chang)的(de)(de)(de)濃度(du)(du)，提高細(xi)(xi)(xi)(xi)胞(bao)(bao)的(de)(de)(de)存活率。溫度(du)(du)控(kong)制(zhi)不(bu)達標慢凍快融是細(xi)(xi)(xi)(xi)胞(bao)(bao)凍存復(fu)蘇(su)的(de)(de)(de)*關(guan)鍵詞之一(yi)適量(liang)地(di)稀釋后，將細(xi)(xi)(xi)(xi)胞(bao)(bao)混合(he)液移至事先準備(bei)好的(de)(de)(de)培養瓶中。南京長(chang)沙(sha)無血清(qing)細(xi)(xi)(xi)(xi)胞(bao)(bao)凍存液

無(wu)血(xue)清細胞凍存(cun)液使用方法：將離(li)心管中(zhong)(zhong)的(de)細胞混合液分(fen)裝(zhuang)于已標示完全的(de)冷凍保存(cun)管中(zhong)(zhong)。蘇州無(wu)血(xue)清細胞凍存(cun)液廠家現貨

凍存(cun)(cun)(cun)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)復蘇方(fang)法：1.從(cong)冰箱里取出(chu)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)冷凍保(bao)存(cun)(cun)(cun)管(guan)(guan)(guan)，立(li)即(ji)放入(ru)37℃振動水浴槽中(zhong)快速解凍。2.待凍存(cun)(cun)(cun)管(guan)(guan)(guan)中(zhong)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)混(hun)合(he)(he)(he)液(ye)完(wan)全融化后，立(li)即(ji)加入(ru)1ml細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)培(pei)(pei)養(yang)(yang)(yang)基(ji)于(yu)該(gai)冷凍管(guan)(guan)(guan)中(zhong)與細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)混(hun)合(he)(he)(he)，再將(jiang)(jiang)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)混(hun)合(he)(he)(he)液(ye)從(cong)凍存(cun)(cun)(cun)管(guan)(guan)(guan)中(zhong)移入(ru)含有9ml該(gai)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)培(pei)(pei)養(yang)(yang)(yang)基(ji)的試管(guan)(guan)(guan)中(zhong)，混(hun)合(he)(he)(he)均勻(yun)。3.離(li)(li)心(xin)收集培(pei)(pei)養(yang)(yang)(yang)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)（參(can)考離(li)(li)心(xin)條件：1,000~2,000rpm，4℃，3~5min），移去上(shang)清液(ye)。4.清洗細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)，充分洗凈殘留凍存(cun)(cun)(cun)液(ye)。5.加入(ru)適(shi)量的新鮮細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)培(pei)(pei)養(yang)(yang)(yang)基(ji)，使用移液(ye)管(guan)(guan)(guan)緩緩地均勻(yun)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)混(hun)合(he)(he)(he)液(ye)。適(shi)量地稀釋后，將(jiang)(jiang)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)混(hun)合(he)(he)(he)液(ye)移至事先(xian)準備好的培(pei)(pei)養(yang)(yang)(yang)瓶中(zhong)。6.鏡檢后，研究者可根據(ju)各(ge)自方(fang)法和需要來進行細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)培(pei)(pei)養(yang)(yang)(yang)。蘇州無血清細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)凍存(cun)(cun)(cun)液(ye)廠家現貨(huo)

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