無血(xue)清(qing)快速細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)凍(dong)存(cun)(cun)(cun)液(ye)(ye)(ye)(ye)，是(shi)一種新型的(de)快速凍(dong)存(cun)(cun)(cun)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)的(de)保護液(ye)(ye)(ye)(ye)，可將細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)置(zhi)于-80^C直接冷凍(dong)保存(cun)(cun)(cun)。NM4100內含天然抗凍(dong)蛋白(Naturalantifrereprotein),不(bu)含動物(wu)來源的(de)血(xue)清(qing)成(cheng)分(fen)，能(neng)夠(gou)有效提高細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)凍(dong)存(cun)(cun)(cun)活率(lv)和(he)復蘇活力，減少各類病毒、霉菌和(he)支(zhi)原體(ti)等的(de)污染，確保凍(dong)存(cun)(cun)(cun)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)安(an)全，能(neng)夠(gou)滿(man)足大部分(fen)體(ti)外培養細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)的(de)凍(dong)存(cun)(cun)(cun)需(xu)求(qiu)。注意事項:請選擇(ze)對數生長(chang)期(qi)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)進行凍(dong)存(cun)(cun)(cun);凍(dong)存(cun)(cun)(cun)液(ye)(ye)(ye)(ye)加入細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)后，請盡快放入-80C冰(bing)箱凍(dong)存(cun)(cun)(cun);3.本(ben)產品凍(dong)存(cun)(cun)(cun)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)可以在-80°C冰(bing)箱保存(cun)(cun)(cun)3年(nian)以上;4.若需(xu)長(chang)期(qi)凍(dong)存(cun)(cun)(cun)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)，請轉移至液(ye)(ye)(ye)(ye)氮罐中(zhong)貯存(cun)(cun)(cun);凍(dong)存(cun)(cun)(cun)液(ye)(ye)(ye)(ye)中(zhong)含DMSO成(cheng)分(fen)，為了您的(de)安(an)全和(he)健康(kang)，請穿(chuan)實驗服并戴一次(ci)性手套。無血(xue)清(qing)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)凍(dong)存(cun)(cun)(cun)液(ye)(ye)(ye)(ye)用于其他類型哺乳動物(wu)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)的(de)儲存(cun)(cun)(cun)。合(he)肥正規(gui)無血(xue)清(qing)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)凍(dong)存(cun)(cun)(cun)液(ye)(ye)(ye)(ye)報價

無(wu)血(xue)清(qing)(qing)細(xi)(xi)胞(bao)(bao)凍(dong)(dong)(dong)(dong)(dong)存(cun)：在細(xi)(xi)胞(bao)(bao)培養中，細(xi)(xi)胞(bao)(bao)凍(dong)(dong)(dong)(dong)(dong)存(cun)是(shi)較關鍵環節之(zhi)一，尤其在細(xi)(xi)胞(bao)(bao)治(zhi)好、細(xi)(xi)胞(bao)(bao)存(cun)儲中對細(xi)(xi)胞(bao)(bao)凍(dong)(dong)(dong)(dong)(dong)存(cun)更有特殊(shu)要求，下(xia)面就根據降(jiang)(jiang)(jiang)(jiang)溫(wen)(wen)(wen)速(su)度以(yi)及凍(dong)(dong)(dong)(dong)(dong)存(cun)液(ye)(ye)(ye)組(zu)分對細(xi)(xi)胞(bao)(bao)凍(dong)(dong)(dong)(dong)(dong)存(cun)做詳細(xi)(xi)介(jie)紹。根據降(jiang)(jiang)(jiang)(jiang)溫(wen)(wen)(wen)速(su)度區(qu)分，細(xi)(xi)胞(bao)(bao)凍(dong)(dong)(dong)(dong)(dong)存(cun)可以(yi)分為(wei)(wei)程序(xu)(xu)降(jiang)(jiang)(jiang)(jiang)溫(wen)(wen)(wen)凍(dong)(dong)(dong)(dong)(dong)存(cun)與非程序(xu)(xu)降(jiang)(jiang)(jiang)(jiang)溫(wen)(wen)(wen)凍(dong)(dong)(dong)(dong)(dong)存(cun)。根據凍(dong)(dong)(dong)(dong)(dong)存(cun)方式不同可以(yi)分為(wei)(wei)慢(man)速(su)凍(dong)(dong)(dong)(dong)(dong)存(cun)（程序(xu)(xu)降(jiang)(jiang)(jiang)(jiang)溫(wen)(wen)(wen)法）與快速(su)凍(dong)(dong)(dong)(dong)(dong)存(cun)（非程序(xu)(xu)降(jiang)(jiang)(jiang)(jiang)溫(wen)(wen)(wen)法）。程序(xu)(xu)降(jiang)(jiang)(jiang)(jiang)溫(wen)(wen)(wen)凍(dong)(dong)(dong)(dong)(dong)存(cun)技術要點(dian)是(shi)慢(man)凍(dong)(dong)(dong)(dong)(dong)，標準的凍(dong)(dong)(dong)(dong)(dong)存(cun)程序(xu)(xu)為(wei)(wei)降(jiang)(jiang)(jiang)(jiang)溫(wen)(wen)(wen)速(su)率-1～-2/℃min；當溫(wen)(wen)(wen)度達-25℃以(yi)下(xia)時，可增至(zhi)-5℃～-10℃/min。之(zhi)前，常用(yong)(yong)的傳統方法是(shi)將(jiang)凍(dong)(dong)(dong)(dong)(dong)存(cun)管(guan)置于(yu)4℃30分鐘--20℃60分鐘--80℃過夜-液(ye)(ye)(ye)氮罐(guan)。不過，由于(yu)步驟較多(duo)，間隔(ge)時間又長，很容易遺忘。之(zhi)后，人們也(ye)開始使用(yong)(yong)程序(xu)(xu)降(jiang)(jiang)(jiang)(jiang)溫(wen)(wen)(wen)盒(he)(he)。將(jiang)凍(dong)(dong)(dong)(dong)(dong)存(cun)管(guan)放入(ru)程序(xu)(xu)降(jiang)(jiang)(jiang)(jiang)溫(wen)(wen)(wen)盒(he)(he)中，再將(jiang)程序(xu)(xu)降(jiang)(jiang)(jiang)(jiang)溫(wen)(wen)(wen)盒(he)(he)放入(ru)-80℃冰箱。以(yi)1℃/min的速(su)度進(jin)行降(jiang)(jiang)(jiang)(jiang)溫(wen)(wen)(wen)。昆(kun)明無(wu)血(xue)清(qing)(qing)細(xi)(xi)胞(bao)(bao)凍(dong)(dong)(dong)(dong)(dong)存(cun)液(ye)(ye)(ye)廠家批(pi)發價無(wu)血(xue)清(qing)(qing)細(xi)(xi)胞(bao)(bao)凍(dong)(dong)(dong)(dong)(dong)存(cun)液(ye)(ye)(ye)不含(han)牛血(xue)清(qing)(qing)，無(wu)動物源組(zu)份(fen)。

無(wu)(wu)血(xue)(xue)清(qing)(qing)快速(su)細(xi)(xi)胞(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)成分。無(wu)(wu)血(xue)(xue)清(qing)(qing)細(xi)(xi)胞(bao)，無(wu)(wu)血(xue)(xue)清(qing)(qing)干(gan)細(xi)(xi)胞(bao)及(ji)MSC凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)。保(bao)(bao)存(cun)(cun)(cun)(cun)條件(jian)：儲存(cun)(cun)(cun)(cun)于(yu)4℃以(yi)下(xia)。質量(liang)保(bao)(bao)障期(qi)從產(chan)品的(de)(de)生產(chan)日(ri)期(qi)起，為期(qi)3年。3個月(yue)以(yi)上沒有使用凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)計劃時，盡(jin)可(ke)能冷凍(dong)(dong)(dong)保(bao)(bao)存(cun)(cun)(cun)(cun)。為避免重(zhong)復(fu)凍(dong)(dong)(dong)融過(guo)程(cheng)可(ke)能導致的(de)(de)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)品質下(xia)降(jiang)和性能降(jiang)低(di)，推薦將100ml產(chan)品分裝小瓶后，再存(cun)(cun)(cun)(cun)放(fang)于(yu)4℃以(yi)下(xia)或-20℃冰箱凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)。無(wu)(wu)血(xue)(xue)清(qing)(qing)細(xi)(xi)胞(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)，細(xi)(xi)胞(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)與(yu)復(fu)蘇后，平均活(huo)(huo)細(xi)(xi)胞(bao)回收比例超過(guo)80%。與(yu)含血(xue)(xue)清(qing)(qing)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)比較，BI無(wu)(wu)血(xue)(xue)清(qing)(qing)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)細(xi)(xi)胞(bao)存(cun)(cun)(cun)(cun)活(huo)(huo)率(lv)都有較佳的(de)(de)表，BI無(wu)(wu)血(xue)(xue)清(qing)(qing)細(xi)(xi)胞(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)也(ye)適用于(yu)動物血(xue)(xue)清(qing)(qing)培養的(de)(de)細(xi)(xi)胞(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)。

細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)凍存(cun)(cun)注意(yi)事項：1、細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)貼壁(bi)(bi)少的(de)(de)(de)(de)問(wen)題：教(jiao)科書中(zhong)說明(ming)凍存(cun)(cun)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)解凍時(shi)1ml細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)液(ye)要加(jia)10ml－15m培(pei)(pei)養液(ye)，而在(zai)(zai)我(wo)的(de)(de)(de)(de)試驗(yan)中(zhong)的(de)(de)(de)(de)經驗(yan)總結為(wei)培(pei)(pei)養基(ji)(ji)(ji)越少細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)越容易貼附。2、復蘇(su)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)分裝的(de)(de)(de)(de)問(wen)題：試驗(yan)中(zhong)我(wo)的(de)(de)(de)(de)經驗(yan)總結為(wei)復蘇(su)1管細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)一般可(ke)分裝到1-2只培(pei)(pei)養瓶中(zhong)，分裝過多，細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)濃(nong)度過低，不利(li)于(yu)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)的(de)(de)(de)(de)貼壁(bi)(bi)。3、加(jia)培(pei)(pei)養基(ji)(ji)(ji)的(de)(de)(de)(de)量放入問(wen)題：這個量的(de)(de)(de)(de)多少的(de)(de)(de)(de)把握(wo)主要涉(she)及到的(de)(de)(de)(de)問(wen)題是DMSO的(de)(de)(de)(de)濃(nong)度，從如(ru)果你加(jia)培(pei)(pei)養基(ji)(ji)(ji)的(de)(de)(de)(de)太(tai)少，那么DMSO的(de)(de)(de)(de)濃(nong)度就會(hui)比較(jiao)大，就會(hui)影響(xiang)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)生長，從以前的(de)(de)(de)(de)資料(liao)來看，DMSO的(de)(de)(de)(de)濃(nong)度在(zai)(zai)小于(yu)0.5%的(de)(de)(de)(de)時(shi)候對(dui)一般細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)沒有(you)什么影響(xiang)，還(huan)有(you)一個說法是1％。所以如(ru)果你的(de)(de)(de)(de)凍存(cun)(cun)液(ye)的(de)(de)(de)(de)濃(nong)度是10％DMSO的(de)(de)(de)(de)話(hua)那么加(jia)10ml以上(shang)的(de)(de)(de)(de)培(pei)(pei)養基(ji)(ji)(ji)就恰好稀釋(shi)到了(le)無(wu)害濃(nong)度無(wu)血清(qing)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)凍存(cun)(cun)液(ye)產品特色：可(ke)直接(jie)存(cun)(cun)放于(yu)-80℃冰箱凍存(cun)(cun)，無(wu)需(xu)經過費時(shi)的(de)(de)(de)(de)程序降溫(wen)過程（省時(shi)、省錢）。

無血(xue)(xue)(xue)清細(xi)(xi)(xi)胞(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)(cun)液(ye)，細(xi)(xi)(xi)胞(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)(cun)與復蘇(su)后，平均活(huo)細(xi)(xi)(xi)胞(bao)(bao)回(hui)收(shou)比(bi)例超過80%。與含血(xue)(xue)(xue)清凍(dong)(dong)(dong)(dong)存(cun)(cun)液(ye)比(bi)較，BI無血(xue)(xue)(xue)清凍(dong)(dong)(dong)(dong)存(cun)(cun)液(ye)細(xi)(xi)(xi)胞(bao)(bao)存(cun)(cun)活(huo)率都有較佳的(de)表(biao)，BI無血(xue)(xue)(xue)清細(xi)(xi)(xi)胞(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)(cun)液(ye)也適(shi)用于動物(wu)血(xue)(xue)(xue)清培(pei)(pei)養(yang)的(de)細(xi)(xi)(xi)胞(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)(cun)。復蘇(su)細(xi)(xi)(xi)胞(bao)(bao)：1.將(jiang)細(xi)(xi)(xi)胞(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)(cun)管由液(ye)態氮中(zhong)取出(chu)，置(zhi)于37度(du)水浴(yu)快速溶解(jie)回(hui)溫。此時(shi)可準(zhun)備培(pei)(pei)養(yang)基(ji)、無菌15ml離(li)(li)心(xin)管、培(pei)(pei)養(yang)瓶。2.于生物(wu)安全柜內，直接(jie)以少量培(pei)(pei)養(yang)基(ji)反(fan)復沖(chong)融，使細(xi)(xi)(xi)胞(bao)(bao)團塊化凍(dong)(dong)(dong)(dong)。3.先(xian)在無菌15ml離(li)(li)心(xin)管中(zhong)加(jia)入5ml培(pei)(pei)養(yang)基(ji)，再將(jiang)化凍(dong)(dong)(dong)(dong)的(de)細(xi)(xi)(xi)胞(bao)(bao)懸液(ye)加(jia)入離(li)(li)心(xin)管中(zhong)。以200~300g，3分鐘離(li)(li)心(xin)收(shou)集(ji)細(xi)(xi)(xi)胞(bao)(bao)。4.倒去上清液(ye)，以新(xin)(xin)鮮培(pei)(pei)養(yang)基(ji)重懸細(xi)(xi)(xi)胞(bao)(bao)，移到培(pei)(pei)養(yang)瓶中(zhong)培(pei)(pei)養(yang)。5.隔天更(geng)換新(xin)(xin)鮮培(pei)(pei)養(yang)基(ji)，并觀察細(xi)(xi)(xi)胞(bao)(bao)存(cun)(cun)活(huo)狀況(kuang)。1000 rmp離(li)(li)心(xin)5分鐘，去除離(li)(li)心(xin)管中(zhong)的(de)上清液(ye)，收(shou)集(ji)細(xi)(xi)(xi)胞(bao)(bao)沉淀(dian)。開(kai)封無血(xue)(xue)(xue)清細(xi)(xi)(xi)胞(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)(cun)液(ye)廠家推薦(jian)

根(gen)據密度調整細(xi)胞凍(dong)(dong)存 液用量。合肥正規無血清細(xi)胞凍(dong)(dong)存液報價

年連續(xu)三年成(cheng)(cheng)為新賽美全產(chan)品線中“受客戶喜歡的科研(yan)產(chan)品”“無(wu)血(xue)清(qing)細胞凍(dong)存液(ye)關鍵技術(shu)及產(chan)業(ye)化”項目成(cheng)(cheng)功入圍“2017年東吳科技創新創業(ye)人才計劃“3優勢分(fen)析(xi)傳統凍(dong)存液(ye)CELLSAVING成(cheng)(cheng)分(fen)“血(xue)清(qing)+培養(yang)基+dmso”四大(da)類，成(cheng)(cheng)分(fen)明確，不(bu)(bu)含(han)血(xue)清(qing)安全性(xing)1、微(wei)(wei)生(sheng)物污染風險(xian)2、批次不(bu)(bu)穩定1、無(wu)微(wei)(wei)生(sheng)物污染風險(xian)險(xian)2、批次穩定操作1、4℃(40min)→20℃(30min)→80℃→液(ye)氮2、程序(xu)性(xing)降溫盒（多人共用(yong)；異丙醇）直接凍(dong)于-80℃冰(bing)箱(xiang)，長期保(bao)存效果(guo)活(huo)率偏低，批次有差異，不(bu)(bu)適用(yong)無(wu)血(xue)清(qing)細胞凍(dong)存90%以上，復蘇后幾乎看不(bu)(bu)到死細胞合肥(fei)正規無(wu)血(xue)清(qing)細胞凍(dong)存液(ye)報價

本文(wen)來自(zi)沈陽喜納多采(cai)暖(nuan)工程有(you)限(xian)公司(si)：//zjlong.cn/Article/29d399950.html