染(ran)色(se)的(de)(de)一(yi)(yi)般原則(ze)：因(yin)檢(jian)(jian)測(ce)細(xi)胞(bao)的(de)(de)類型、凋亡誘導劑(ji)種類、使(shi)(shi)用(yong)的(de)(de)檢(jian)(jian)測(ce)儀器不(bu)同(tong)，因(yin)而(er)流式檢(jian)(jian)測(ce)的(de)(de)熒(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)補償也不(bu)同(tong)，因(yin)此(ci)建議每次(ci)(ci)檢(jian)(jian)測(ce)均需使(shi)(shi)用(yong)凋亡誘導處理的(de)(de)細(xi)胞(bao)做單(dan)陽對照，進行(xing)熒(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)補償的(de)(de)調(diao)節。標記抗體常用(yong)熒(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)素FITC，EGFP激(ji)發(fa)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)：488nm發(fa)射(she)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)：525nm;使(shi)(shi)用(yong)面較(jiao)廣，價格便宜。PE激(ji)發(fa)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)488nm發(fa)射(she)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)575nm，此(ci)熒(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)的(de)(de)激(ji)發(fa)效率較(jiao)佳，故此(ci)熒(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)得到的(de)(de)信號較(jiao)強；檢(jian)(jian)測(ce)某(mou)些弱表(biao)達(da)的(de)(de)抗原，推薦使(shi)(shi)用(yong)此(ci)熒(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)素。價格較(jiao)FITC昂貴。APC激(ji)發(fa)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)633nm，發(fa)射(she)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)660nm，在配有(you)雙激(ji)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)的(de)(de)流式細(xi)胞(bao)儀上，此(ci)熒(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)染(ran)料(liao)可與7-AAD或PI共(gong)同(tong)使(shi)(shi)用(yong)來(lai)避開自(zi)帶(dai)綠色(se)熒(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)的(de)(de)樣本。流式細(xi)胞(bao)儀相關(guan)試劑(ji)盒(he)。為了您的(de)(de)安(an)全和健康，請穿實驗服并戴一(yi)(yi)次(ci)(ci)性手(shou)套操作(zuo)。天津如何使(shi)(shi)用(yong)糖原染(ran)色(se)試劑(ji)盒(he)量(liang)大從(cong)優(you)

糖(tang)(tang)原(yuan)(yuan)染(ran)色正常值:正常情況下(xia)，紅(hong)細(xi)(xi)(xi)(xi)胞(bao)(bao)系(xi)統(tong)(tong)的(de)原(yuan)(yuan)、幼紅(hong)細(xi)(xi)(xi)(xi)胞(bao)(bao)和(he)成熟(shu)紅(hong)細(xi)(xi)(xi)(xi)胞(bao)(bao);粒細(xi)(xi)(xi)(xi)胞(bao)(bao)系(xi)統(tong)(tong)的(de)原(yuan)(yuan)粒細(xi)(xi)(xi)(xi)胞(bao)(bao)、原(yuan)(yuan)單核細(xi)(xi)(xi)(xi)胞(bao)(bao)和(he)大多數淋(lin)巴細(xi)(xi)(xi)(xi)胞(bao)(bao)為陰(yin)性(xing)(xing)(xing)(xing)(xing)(xing)反應(ying)。自早(zao)幼粒階段以后的(de)粒細(xi)(xi)(xi)(xi)胞(bao)(bao)和(he)幼單核細(xi)(xi)(xi)(xi)胞(bao)(bao)可呈(cheng)弱(ruo)陽性(xing)(xing)(xing)(xing)(xing)(xing)反應(ying)。糖(tang)(tang)原(yuan)(yuan)染(ran)色臨床意義:(1)急(ji)性(xing)(xing)(xing)(xing)(xing)(xing)淋(lin)巴細(xi)(xi)(xi)(xi)胞(bao)(bao)白(bai)(bai)血(xue)(xue)(xue)病、淋(lin)巴組(zu)織惡(e)性(xing)(xing)(xing)(xing)(xing)(xing)增生(sheng)(sheng)性(xing)(xing)(xing)(xing)(xing)(xing)疾病、紅(hong)白(bai)(bai)血(xue)(xue)(xue)病、戈謝病的(de)原(yuan)(yuan)始細(xi)(xi)(xi)(xi)胞(bao)(bao)呈(cheng)強陽性(xing)(xing)(xing)(xing)(xing)(xing)反應(ying)或(huo)陽性(xing)(xing)(xing)(xing)(xing)(xing)反應(ying);缺鐵(tie)性(xing)(xing)(xing)(xing)(xing)(xing)貧(pin)血(xue)(xue)(xue)、珠蛋白(bai)(bai)生(sheng)(sheng)成障(zhang)礙(ai)、骨(gu)髓增生(sheng)(sheng)異常綜合(he)征亦可呈(cheng)陽性(xing)(xing)(xing)(xing)(xing)(xing)反應(ying);急(ji)性(xing)(xing)(xing)(xing)(xing)(xing)粒細(xi)(xi)(xi)(xi)胞(bao)(bao)白(bai)(bai)血(xue)(xue)(xue)病、急(ji)性(xing)(xing)(xing)(xing)(xing)(xing)單核細(xi)(xi)(xi)(xi)胞(bao)(bao)白(bai)(bai)血(xue)(xue)(xue)病、良性(xing)(xing)(xing)(xing)(xing)(xing)淋(lin)巴細(xi)(xi)(xi)(xi)胞(bao)(bao)增多癥、尼曼-皮克細(xi)(xi)(xi)(xi)胞(bao)(bao)呈(cheng)陰(yin)性(xing)(xing)(xing)(xing)(xing)(xing)反應(ying)或(huo)弱(ruo)陽性(xing)(xing)(xing)(xing)(xing)(xing)反應(ying)。巨幼細(xi)(xi)(xi)(xi)胞(bao)(bao)性(xing)(xing)(xing)(xing)(xing)(xing)貧(pin)血(xue)(xue)(xue)、溶血(xue)(xue)(xue)性(xing)(xing)(xing)(xing)(xing)(xing)貧(pin)血(xue)(xue)(xue)、再生(sheng)(sheng)障(zhang)礙(ai)性(xing)(xing)(xing)(xing)(xing)(xing)貧(pin)血(xue)(xue)(xue)等，幼紅(hong)細(xi)(xi)(xi)(xi)胞(bao)(bao)為陰(yin)性(xing)(xing)(xing)(xing)(xing)(xing)反應(ying)。偶有個別幼紅(hong)細(xi)(xi)(xi)(xi)胞(bao)(bao)呈(cheng)陽性(xing)(xing)(xing)(xing)(xing)(xing)反應(ying)。(2)幫(bang)助鑒(jian)(jian)別不(bu)典型巨核細(xi)(xi)(xi)(xi)胞(bao)(bao)和(he)霍奇金細(xi)(xi)(xi)(xi)胞(bao)(bao)，巨核細(xi)(xi)(xi)(xi)胞(bao)(bao)呈(cheng)強陽性(xing)(xing)(xing)(xing)(xing)(xing)反應(ying);霍奇金細(xi)(xi)(xi)(xi)胞(bao)(bao)呈(cheng)弱(ruo)陽性(xing)(xing)(xing)(xing)(xing)(xing)或(huo)陰(yin)性(xing)(xing)(xing)(xing)(xing)(xing)反應(ying)。(3)幫(bang)助鑒(jian)(jian)別白(bai)(bai)血(xue)(xue)(xue)病細(xi)(xi)(xi)(xi)胞(bao)(bao)和(he)腺(xian)骨(gu)髓轉移的(de)腺(xian)細(xi)(xi)(xi)(xi)胞(bao)(bao)，腺(xian)細(xi)(xi)(xi)(xi)胞(bao)(bao)呈(cheng)陽性(xing)(xing)(xing)(xing)(xing)(xing)反應(ying)。口(kou)碑(bei)好的(de)糖(tang)(tang)原(yuan)(yuan)染(ran)色試劑盒哪(na)家便宜糖(tang)(tang)尿(niao)病性(xing)(xing)(xing)(xing)(xing)(xing)腎小球硬化(hua)癥或(huo)血(xue)(xue)(xue)管病的(de)診斷。

糖原(yuan)染(ran)(ran)色(se)是病(bing)理學中(zhong)(zhong)常規(gui)的(de)染(ran)(ran)色(se)方法之(zhi)一，McManus在1946年較先(xian)使(shi)用高碘(dian)酸(suan)-雪夫技術顯(xian)示黏蛋白，該法常用來顯(xian)示糖原(yuan)和其他(ta)多(duo)糖，該染(ran)(ran)色(se)試劑盒不僅能夠顯(xian)示糖原(yuan)，還(huan)能顯(xian)示中(zhong)(zhong)性黏液性物(wu)質(zhi)和某(mou)些酸(suan)性物(wu)質(zhi)以及軟(ruan)骨、垂體、霉菌、色(se)素、淀粉(fen)樣物(wu)質(zhi)、基(ji)(ji)底膜等。過(guo)(guo)碘(dian)酸(suan)(又稱高碘(dian)酸(suan))是一種強氧(yang)(yang)化(hua)劑，它能氧(yang)(yang)化(hua)糖類及有關物(wu)質(zhi)中(zhong)(zhong)的(de)1，2-乙(yi)二(er)醇基(ji)(ji)，使(shi)之(zhi)變(bian)為二(er)醛，醛不Schiff試劑能結(jie)合(he)(he)成(cheng)一種品紅(hong)(hong)化(hua)合(he)(he)物(wu)，產生紫紅(hong)(hong)色(se)。由于高碘(dian)酸(suan)還(huan)可氧(yang)(yang)化(hua)細胞內其他(ta)物(wu)質(zhi)，使(shi)用時應注意選擇好高碘(dian)酸(suan)濃度和氧(yang)(yang)化(hua)時間，使(shi)氧(yang)(yang)化(hua)控制(zhi)在既能把乙(yi)二(er)醇基(ji)(ji)氧(yang)(yang)化(hua)成(cheng)醛基(ji)(ji)又不至于過(guo)(guo)氧(yang)(yang)化(hua)，這(zhe)是很關鍵的(de)步驟。

粘(zhan)液(ye)(ye)染(ran)(ran)(ran)色(se)法：粘(zhan)液(ye)(ye)一(yi)般有以下幾點(dian)特性(xing)(xing)(xing)：（1）對(dui)鹽(yan)基性(xing)(xing)(xing)染(ran)(ran)(ran)料有親(qin)合(he)力，因此在HE染(ran)(ran)(ran)色(se)中(zhong)、切片(pian)上的粘(zhan)液(ye)(ye)呈污穢藍色(se)。（2）對(dui)某種鹽(yan)基性(xing)(xing)(xing)人工(gong)合(he)成染(ran)(ran)(ran)料如硫堇或甲苯胺藍有異染(ran)(ran)(ran)性(xing)(xing)(xing)。（3）遇醋(cu)酸發生沉淀，胃粘(zhan)蛋白則除外。（4）溶(rong)于弱堿(jian)溶(rong)液(ye)(ye)。常(chang)(chang)(chang)用的粘(zhan)液(ye)(ye)染(ran)(ran)(ran)色(se)有以下幾種：1、P．A．S染(ran)(ran)(ran)色(se)法：操(cao)作(zuo)方法同前，結果(guo)；中(zhong)性(xing)(xing)(xing)粘(zhan)液(ye)(ye)性(xing)(xing)(xing)物質，某些酸性(xing)(xing)(xing)粘(zhan)液(ye)(ye)物質呈紅(hong)(hong)色(se)，核(he)呈藍色(se)。2、AB/P.A.S染(ran)(ran)(ran)色(se)法：該種方法的特點(dian)是能同時(shi)顯(xian)示出(chu)酸性(xing)(xing)(xing)和中(zhong)性(xing)(xing)(xing)粘(zhan)液(ye)(ye)物質。操(cao)作(zuo)方法：（1）切片(pian)常(chang)(chang)(chang)規(gui)脫蠟入水(shui)。（2）3%醋(cu)酸液(ye)(ye)洗(xi)(xi)2分(fen)鐘，入1%阿(a)利(li)新蘭醋(cu)酸液(ye)(ye)（PH2.5）10-20分(fen)鐘。（3）蒸餾(liu)水(shui)洗(xi)(xi)。（4）按P.A.S染(ran)(ran)(ran)色(se)程序。（5）蘇(su)木素淡染(ran)(ran)(ran)2-3分(fen)鐘，水(shui)洗(xi)(xi)。（6）常(chang)(chang)(chang)規(gui)脫水(shui)、封(feng)片(pian)。巨幼(you)(you)紅(hong)(hong)細胞貧(pin)(pin)血、溶(rong)血性(xing)(xing)(xing)貧(pin)(pin)血、再障(zhang)的幼(you)(you)紅(hong)(hong)細胞PAS染(ran)(ran)(ran)色(se)為(wei)陰性(xing)(xing)(xing)。

染(ran)色(se)試(shi)劑盒：GUS基(ji)因(yin)(yin)就是β-葡萄糖(tang)苷(gan)(gan)(gan)酸酶(mei)基(ji)因(yin)(yin)(β-glucuronidase)，是從大(da)腸桿菌K-12菌株分(fen)(fen)離(li)出(chu)的一(yi)種(zhong)(zhong)酸性水解酶(mei)。該(gai)基(ji)因(yin)(yin)產物(wu)為(wei)β-葡萄糖(tang)苷(gan)(gan)(gan)酸酶(mei)，屬水解酶(mei)類，相當穩定而不易降解，以β-葡萄糖(tang)苷(gan)(gan)(gan)酸酯類物(wu)質(zhi)為(wei)底物(wu)，其反應產物(wu)可用(yong)多種(zhong)(zhong)方法檢測(ce)出(chu)來。由(you)于絕大(da)多數(shu)植(zhi)物(wu)沒有檢測(ce)到葡萄糖(tang)苷(gan)(gan)(gan)酸酶(mei)的背景活性，因(yin)(yin)此這個基(ji)因(yin)(yin)被(bei)普遍應用(yong)于基(ji)因(yin)(yin)調控(kong)的研究中。根據GUS基(ji)因(yin)(yin)檢測(ce)所用(yong)的底物(wu)不同(tong)，可以選擇三種(zhong)(zhong)檢測(ce)方法：組織(zhi)化學法、分(fen)(fen)光(guang)光(guang)度法和熒光(guang)法（靈感(gan)度為(wei)分(fen)(fen)光(guang)光(guang)度檢測(ce)法較高）。其中較為(wei)常(chang)用(yong)的是組織(zhi)化學法。如用(yong)無水酒精作(zuo)糖(tang)原的固定劑，有它的弊病(bing)。福建糖(tang)原染(ran)色(se)試(shi)劑盒哪(na)家便宜

切片脫蠟(la)應盡量(liang)干(gan)凈，否則影響染(ran)色效果。天津如(ru)何(he)使用糖原(yuan)染(ran)色試劑盒量(liang)大從(cong)優

糖(tang)(tang)(tang)原(yuan)(yuan)染色(se)(過碘酸-雪(xue)夫(fu)反(fan)應(ying))原(yuan)(yuan)理多糖(tang)(tang)(tang)中(zhong)(zhong)乙二醇基經過碘酸氧(yang)化成(cheng)(cheng)二醛基與無(wu)色(se)品紅(hong)(hong)結(jie)合，形成(cheng)(cheng)紫(zi)紅(hong)(hong)色(se)化合物。沉(chen)積(ji)于含糖(tang)(tang)(tang)原(yuan)(yuan)的(de)胞(bao)(bao)漿(jiang)中(zhong)(zhong)。結(jie)果：胞(bao)(bao)漿(jiang)中(zhong)(zhong)出現彌(mi)散狀，顆(ke)粒狀，塊(kuai)(kuai)狀紅(hong)(hong)色(se)為(wei)陽(yang)性(xing)(xing)。無(wu)紅(hong)(hong)色(se)顆(ke)粒為(wei)陰性(xing)(xing)_x005f_x000c_臨床意義(yi)1、正(zheng)(zheng)常(chang)血(xue)細(xi)(xi)(xi)胞(bao)(bao)的(de)染色(se)反(fan)應(ying)粒系：原(yuan)(yuan)粒細(xi)(xi)(xi)胞(bao)(bao)糖(tang)(tang)(tang)原(yuan)(yuan)含量很(hen)低(di)，隨著細(xi)(xi)(xi)胞(bao)(bao)的(de)分化成(cheng)(cheng)熟，糖(tang)(tang)(tang)原(yuan)(yuan)含量逐增加(jia)，至成(cheng)(cheng)熟階段糖(tang)(tang)(tang)原(yuan)(yuan)含量十分豐富。淋巴細(xi)(xi)(xi)胞(bao)(bao)：糖(tang)(tang)(tang)原(yuan)(yuan)常(chang)凝聚成(cheng)(cheng)顆(ke)粒或塊(kuai)(kuai)狀。巨核(he)細(xi)(xi)(xi)胞(bao)(bao)－血(xue)小(xiao)板系統：PAS反(fan)應(ying)呈粗大的(de)紫(zi)色(se)顆(ke)粒或團塊(kuai)(kuai)。正(zheng)(zheng)常(chang)紅(hong)(hong)系：陰性(xing)(xing)。對(dui)疾病診(zhen)斷(duan)的(de)意義(yi)白(bai)(bai)血(xue)病類型(xing)的(de)鑒別(bie)：急(ji)性(xing)(xing)粒細(xi)(xi)(xi)胞(bao)(bao)白(bai)(bai)血(xue)病：原(yuan)(yuan)始(shi)粒細(xi)(xi)(xi)胞(bao)(bao)呈陰性(xing)(xing)或弱陽(yang)性(xing)(xing)反(fan)應(ying)，以彌(mi)散性(xing)(xing)為(wei)主；急(ji)性(xing)(xing)淋巴細(xi)(xi)(xi)胞(bao)(bao)白(bai)(bai)血(xue)病原(yuan)(yuan)、幼(you)淋巴細(xi)(xi)(xi)胞(bao)(bao)多呈粗顆(ke)粒、塊(kuai)(kuai)狀陽(yang)性(xing)(xing)反(fan)應(ying)天(tian)津如何使用糖(tang)(tang)(tang)原(yuan)(yuan)染色(se)試劑盒量大從優

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