糖(tang)原染(ran)色――檢查項目的(de)注意(yi)細節：其(qi)判斷標準隨(sui)細胞不(bu)同而異，一般分(fen)為5級，即(ji)0～4級。糖(tang)原染(ran)色――檢查項目的(de)一般步驟(zou)：(1)新鮮干(gan)燥(zao)血(xue)片或(huo)骨髓片于(yu)95%乙(yi)醇中固定(ding)10min，蒸(zheng)(zheng)餾(liu)水(shui)(shui)(shui)沖(chong)洗數(shu)次(ci)，待干(gan)。(2)浸入(ru)10g/L過碘(dian)酸水(shui)(shui)(shui)溶液(ye)中10min，蒸(zheng)(zheng)餾(liu)水(shui)(shui)(shui)沖(chong)洗數(shu)次(ci)，待完(wan)全(quan)干(gan)燥(zao)。(3)放(fang)入(ru)雪(xue)夫(fu)液(ye)30min，用(yong)自(zi)(zi)來水(shui)(shui)(shui)沖(chong)洗約5min，再(zai)用(yong)蒸(zheng)(zheng)餾(liu)水(shui)(shui)(shui)沖(chong)洗，然后用(yong)20g/L甲(jia)基綠(lv)復染(ran)20min，水(shui)(shui)(shui)洗，晾干(gan)。糖(tang)原染(ran)色――檢查項目結果解讀：(1)新鮮干(gan)燥(zao)血(xue)片或(huo)骨髓片于(yu)95%乙(yi)醇中固定(ding)10min，蒸(zheng)(zheng)餾(liu)水(shui)(shui)(shui)沖(chong)洗數(shu)次(ci)，待干(gan)。(2)浸入(ru)10g/L過碘(dian)酸水(shui)(shui)(shui)溶液(ye)中10min，蒸(zheng)(zheng)餾(liu)水(shui)(shui)(shui)沖(chong)洗數(shu)次(ci)，待完(wan)全(quan)干(gan)燥(zao)。(3)放(fang)入(ru)雪(xue)夫(fu)液(ye)30min，用(yong)自(zi)(zi)來水(shui)(shui)(shui)沖(chong)洗約5min，再(zai)用(yong)蒸(zheng)(zheng)餾(liu)水(shui)(shui)(shui)沖(chong)洗，然后用(yong)20g/L甲(jia)基綠(lv)復染(ran)20min，水(shui)(shui)(shui)洗，晾干(gan)。自(zi)(zi)早(zao)幼(you)粒階段以(yi)后的(de)粒細胞和幼(you)單核細胞可呈弱陽性反應。北京(jing)如何使用(yong)糖(tang)原染(ran)色試劑盒(he)平均價格

糖(tang)(tang)(tang)(tang)(tang)(tang)類染(ran)(ran)色(se)(se)：糖(tang)(tang)(tang)(tang)(tang)(tang)類從(cong)組織化學技(ji)術角度來分，可(ke)分為(wei)(wei)(wei)(wei)多(duo)糖(tang)(tang)(tang)(tang)(tang)(tang)、中(zhong)性(xing)粘(zhan)液(ye)物質(zhi)、酸(suan)性(xing)粘(zhan)液(ye)物質(zhi)、粘(zhan)蛋白及(ji)粘(zhan)脂質(zhi)。多(duo)糖(tang)(tang)(tang)(tang)(tang)(tang)主要指糖(tang)(tang)(tang)(tang)(tang)(tang)原(yuan)(yuan)(yuan)(yuan)，它是(shi)一種膠樣液(ye)態存(cun)在(zai)(zai)于(yu)肝(gan)細胞(bao)、骨(gu)骼肌(ji)、心肌(ji)等處(chu)。因糖(tang)(tang)(tang)(tang)(tang)(tang)原(yuan)(yuan)(yuan)(yuan)在(zai)(zai)肝(gan)臟及(ji)心肌(ji)疾患及(ji)某些的(de)病(bing)變中(zhong)分布和量都有(you)一定(ding)改(gai)變，故糖(tang)(tang)(tang)(tang)(tang)(tang)原(yuan)(yuan)(yuan)(yuan)的(de)染(ran)(ran)色(se)(se)在(zai)(zai)臨床病(bing)理診(zhen)斷(duan)(duan)上(shang)具有(you)重要意義。（1）肝(gan)及(ji)心肌(ji)糖(tang)(tang)(tang)(tang)(tang)(tang)原(yuan)(yuan)(yuan)(yuan)沉著癥的(de)診(zhen)斷(duan)(duan)：糖(tang)(tang)(tang)(tang)(tang)(tang)原(yuan)(yuan)(yuan)(yuan)染(ran)(ran)色(se)(se)顯示在(zai)(zai)肝(gan)或心肌(ji)細胞(bao)內有(you)大量糖(tang)(tang)(tang)(tang)(tang)(tang)原(yuan)(yuan)(yuan)(yuan)存(cun)在(zai)(zai)即(ji)可(ke)確診(zhen)。（2）糖(tang)(tang)(tang)(tang)(tang)(tang)尿病(bing)性(xing)腎小球(qiu)硬化癥或血管病(bing)的(de)診(zhen)斷(duan)(duan)。（3）高雪氏(shi)病(bing)與尼曼(man)——匹(pi)克氏(shi)病(bing)的(de)鑒別：前者(zhe)為(wei)(wei)(wei)(wei)陽(yang)性(xing)、后者(zhe)為(wei)(wei)(wei)(wei)陰性(xing)，做此(ci)染(ran)(ran)色(se)(se)較(jiao)易鑒別。（4）骨(gu)內骨(gu)外尤(you)文(wen)氏(shi)肉瘤(liu)的(de)診(zhen)斷(duan)(duan)：此(ci)瘤(liu)80%細胞(bao)內有(you)較(jiao)多(duo)糖(tang)(tang)(tang)(tang)(tang)(tang)原(yuan)(yuan)(yuan)(yuan)，糖(tang)(tang)(tang)(tang)(tang)(tang)原(yuan)(yuan)(yuan)(yuan)染(ran)(ran)色(se)(se)為(wei)(wei)(wei)(wei)陽(yang)性(xing)，所以(yi)糖(tang)(tang)(tang)(tang)(tang)(tang)原(yuan)(yuan)(yuan)(yuan)染(ran)(ran)色(se)(se)為(wei)(wei)(wei)(wei)陽(yang)性(xing)（大多(duo)數細胞(bao)）可(ke)以(yi)協助診(zhen)斷(duan)(duan)。（5）腺泡狀軟組織肉瘤(liu)與化感瘤(liu)：前者(zhe)棒狀小體糖(tang)(tang)(tang)(tang)(tang)(tang)原(yuan)(yuan)(yuan)(yuan)染(ran)(ran)色(se)(se)為(wei)(wei)(wei)(wei)陽(yang)性(xing)。福(fu)建正規糖(tang)(tang)(tang)(tang)(tang)(tang)原(yuan)(yuan)(yuan)(yuan)染(ran)(ran)色(se)(se)試(shi)劑盒平(ping)均價格加入少量活性(xing)碳并(bing)震蕩(dang)、過濾，此(ci)時(shi)溶液(ye)應為(wei)(wei)(wei)(wei)無色(se)(se)。

糖原(yuan)D-PAS染色(se)液(ye)：使(shi)用(yong)方法：1、兩(liang)張相同切片，二(er)甲(jia)(jia)苯脫蠟，梯度(du)乙醇入(ru)水(shui)。2、一張切片入(ru)37℃淀粉酶溶(rong)液(ye)處(chu)(chu)理1h。另一張不用(yong)淀粉酶溶(rong)液(ye)處(chu)(chu)理，入(ru)水(shui)中1h作為對照(zhao)。3、流水(shui)沖(chong)洗兩(liang)張切片各(ge)5～10min。4、入(ru)過碘(dian)酸溶(rong)液(ye)，室溫(wen)放置5～8min，一般不宜(yi)超(chao)過10min。5、自來(lai)水(shui)沖(chong)洗1次，再(zai)用(yong)蒸(zheng)餾水(shui)浸(jin)洗2次。6、入(ru)SchiffReagent，置于室溫(wen)陰(yin)暗處(chu)(chu)浸(jin)染10～20min。7、自來(lai)水(shui)沖(chong)洗10min。8、入(ru)Mayer蘇木素(su)染色(se)液(ye)，染細胞核1～2min。9、(可選)酸性乙醇分(fen)化液(ye)分(fen)化2～5s。10、自來(lai)水(shui)沖(chong)洗10～15min，更換(huan)蒸(zheng)餾水(shui)清(qing)洗使(shi)其返藍。11、二(er)甲(jia)(jia)苯透明，中性樹膠(jiao)封固。

糖(tang)(tang)原染色在(zai)(zai)使(shi)用(yong)(yong)(yong)過程中(zhong)需要注意以下幾個(ge)方面：盡可能(neng)(neng)選(xuan)取(qu)小塊(kuai)新(xin)鮮組織(zhi)(zhi)及時(shi)固(gu)定(ding)。糖(tang)(tang)原易(yi)溶(rong)(rong)(rong)于(yu)水(shui)，在(zai)(zai)酶(mei)的作用(yong)(yong)(yong)下很容易(yi)分(fen)解葡萄糖(tang)(tang)，更易(yi)溶(rong)(rong)(rong)于(yu)水(shui)，固(gu)定(ding)之前決不(bu)能(neng)(neng)用(yong)(yong)(yong)水(shui)或生理鹽水(shui)等浸(jin)洗。應避(bi)免用(yong)(yong)(yong)水(shui)溶(rong)(rong)(rong)性(xing)固(gu)定(ding)液(ye)(ye)，宜采用(yong)(yong)(yong)酒精(jing)性(xing)固(gu)定(ding)液(ye)(ye)，如Carnoy液(ye)(ye)，能(neng)(neng)較(jiao)好地保存(cun)糖(tang)(tang)原，但有使(shi)糖(tang)(tang)原流動到細(xi)胞一側的現象，多數(shu)人認為是由于(yu)固(gu)定(ding)劑把細(xi)胞內(nei)的糖(tang)(tang)原推向(xiang)固(gu)定(ding)劑對(dui)組織(zhi)(zhi)浸(jin)透的方向(xiang)而造成。其他組織(zhi)(zhi)應用(yong)(yong)(yong)中(zhong)性(xing)福爾馬(ma)林固(gu)定(ding)為佳。組織(zhi)(zhi)在(zai)(zai)4℃左右溫度內(nei)固(gu)定(ding)與保存(cun)，是針對(dui)糖(tang)(tang)的性(xing)質(zhi)和(he)避(bi)免糖(tang)(tang)原溶(rong)(rong)(rong)于(yu)水(shui)而采取(qu)的相(xiang)應措施(shi)。乙醇(chun)能(neng)(neng)沉淀白蛋白、球(qiu)蛋白，亦能(neng)(neng)白和(he)糖(tang)(tang)原沉淀，但仍可溶(rong)(rong)(rong)于(yu)水(shui)，因此較(jiao)好不(bu)單(dan)獨使(shi)用(yong)(yong)(yong)乙醇(chun)固(gu)定(ding)，以乙醇(chun)為溶(rong)(rong)(rong)劑的混(hun)合(he)固(gu)定(ding)液(ye)(ye)固(gu)定(ding)為佳推薦(jian)用(yong)(yong)(yong)于(yu)骨(gu)和(he)軟骨(gu)的染色。

糖(tang)原(yuan)染(ran)色(se)(se)在使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)過(guo)程中需(xu)要注(zhu)意(yi)以(yi)下幾個(ge)方面(mian)：Schiff氏(shi)染(ran)液的(de)(de)(de)配(pei)(pei)(pei)(pei)制(zhi)是(shi)關(guan)鍵，尤(you)其是(shi)偏重(zhong)亞硫酸鈉的(de)(de)(de)質(zhi)量，打開(kai)應(ying)(ying)是(shi)粉劑，且帶有硫的(de)(de)(de)氣(qi)味，若成(cheng)團或(huo)沒(mei)有氣(qi)味，則不能用(yong)(yong)(yong)(yong)，配(pei)(pei)(pei)(pei)制(zhi)時(shi)(shi)(shi)(shi)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)的(de)(de)(de)容器、藥勺、稱(cheng)藥天平與(yu)稱(cheng)藥紙等必須(xu)潔凈、無(wu)污染(ran)。新配(pei)(pei)(pei)(pei)制(zhi)好(hao)的(de)(de)(de)Schiff劑為無(wu)色(se)(se)透(tou)明液體(ti)[1]，配(pei)(pei)(pei)(pei)好(hao)后可分成(cheng)小份(fen)置于-20℃保存(cun)，用(yong)(yong)(yong)(yong)時(shi)(shi)(shi)(shi)取(qu)一(yi)(yi)份(fen)恢(hui)復到室(shi)溫即可。沈洪武等通過(guo)對(dui)比染(ran)色(se)(se)發現(xian)，冷凍保存(cun)1年的(de)(de)(de)Schiff液的(de)(de)(de)染(ran)色(se)(se)結(jie)果(guo)與(yu)新鮮(xian)配(pei)(pei)(pei)(pei)制(zhi)的(de)(de)(de)染(ran)色(se)(se)結(jie)果(guo)一(yi)(yi)致(zhi)[5]。0.5％～1％高(gao)碘(dian)酸（pH值(zhi)在3.0～5.0之間(jian)），環境(jing)溫度以(yi)不高(gao)于20℃為宜，室(shi)溫高(gao)時(shi)(shi)(shi)(shi)氧化時(shi)(shi)(shi)(shi)間(jian)適(shi)當縮短(duan)；如(ru)果(guo)染(ran)色(se)(se)時(shi)(shi)(shi)(shi)環境(jing)溫度較(jiao)高(gao)且高(gao)碘(dian)酸作用(yong)(yong)(yong)(yong)時(shi)(shi)(shi)(shi)間(jian)長，可使(shi)(shi)(shi)某些物(wu)質(zhi)成(cheng)分發生(sheng)非特異反(fan)應(ying)(ying)，使(shi)(shi)(shi)染(ran)色(se)(se)結(jie)果(guo)出現(xian)假(jia)陽性。因(yin)此，室(shi)溫較(jiao)高(gao)時(shi)(shi)(shi)(shi)可適(shi)當縮短(duan)反(fan)應(ying)(ying)時(shi)(shi)(shi)(shi)間(jian)。取(qu)材必須(xu)新鮮(xian)，這一(yi)(yi)點對(dui)于從事細胞生(sheng)物(wu)學(xue)研究尤(you)為重(zhong)要。北京如(ru)何使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)糖(tang)原(yuan)染(ran)色(se)(se)試劑盒平均價格

組織(zhi)石蠟切(qie)片的染色(se)檢(jian)測。北(bei)京如(ru)何使用(yong)糖原染色(se)試劑盒平(ping)均價(jia)格

特(te)染(ran)(ran)的(de)應用(yong)(yong)價(jia)(jia)值：現(xian)代病(bing)理學中(zhong)(zhong)免疫組(zu)(zu)織(zhi)化(hua)學技(ji)術(shu)(shu)、電子顯微(wei)鏡技(ji)術(shu)(shu)以(yi)(yi)及(ji)其它細胞及(ji)分子生物(wu)學技(ji)術(shu)(shu)應用(yong)(yong)日益(yi)普遍，但由于這些(xie)技(ji)術(shu)(shu)要(yao)求一定的(de)實(shi)驗(yan)條(tiao)件以(yi)(yi)及(ji)所需的(de)試(shi)劑(ji)價(jia)(jia)格較為(wei)(wei)(wei)昂(ang)貴，對(dui)于一部分病(bing)人以(yi)(yi)及(ji)某一些(xie)基層醫(yi)院是比較難以(yi)(yi)接受的(de)。而組(zu)(zu)織(zhi)化(hua)學技(ji)術(shu)(shu)則具(ju)有(you)無需復雜(za)的(de)實(shi)驗(yan)條(tiao)件以(yi)(yi)及(ji)較為(wei)(wei)(wei)昂(ang)貴的(de)試(shi)劑(ji)操作又比較簡(jian)單(dan)的(de)優(you)勢(shi)，在臨床(chuang)病(bing)理學診斷中(zhong)(zhong)具(ju)有(you)重要(yao)的(de)應用(yong)(yong)價(jia)(jia)值。比如(ru)，當(dang)細胞中(zhong)(zhong)出現(xian)色素，是黑色素還是含鐵血(xue)黃(huang)素以(yi)(yi)及(ji)當(dang)組(zu)(zu)織(zhi)中(zhong)(zhong)出現(xian)均(jun)一化(hua)學物(wu)質是否為(wei)(wei)(wei)淀粉樣變性等(deng)，用(yong)(yong)組(zu)(zu)織(zhi)化(hua)學技(ji)術(shu)(shu)區別起來很簡(jian)單(dan)，所以(yi)(yi)組(zu)(zu)織(zhi)化(hua)學技(ji)術(shu)(shu)，雖然已(yi)有(you)幾(ji)(ji)十年到幾(ji)(ji)百年的(de)歷史，仍是一個很有(you)實(shi)用(yong)(yong)價(jia)(jia)值的(de)技(ji)術(shu)(shu)北京如(ru)何使用(yong)(yong)糖原染(ran)(ran)色試(shi)劑(ji)盒平(ping)均(jun)價(jia)(jia)格

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